April, 9, 2013
March 18, 2013
Birth of Ubiquitin-NLS-GFP
March 13, 2013
Birth of Ubiquitin-dsTomato
PNAS manuscript accepted details coming soon
Nov 15, 2012
Swine Genome Complete
Link to the companion papers
Oct 1, 2012
Marker Free hDAF-into-GGTA1 Pig
June 8, 2012
Pigs used in Microbiome Studies
May 21, 2012
Birth of New Cancer Model Piglets
April 16, 2012
Birth of New Piglets:
2) Liver Specific Suicide Gene
April 1, 2012
Launch of a New/Updated Website
March 1, 2012
Generation of an inbred miniature pig model of retinitis pigmentosa.
Ross JW, Fernandez de Castro JP, Zhao J, Samuel M, Walters E, Rios C, Bray-Ward P, Jones BW, Marc RE, Wang W, Zhou L, Noel JM, McCall MA, Demarco PJ, Prather RS, Kaplan HJ.
Invest Ophthalmol Vis Sci. 2012 Jan 31;53(1):501-7. Print 2012 Jan.
K Lee, R.S. Prather. 2013. Cloning pigs by somatic cell nuclear transfer. In: Principles of Cloning, 2nd edition (J. Cibelli, J. Gurdon, I. Wilmut, R. Jaenisch, R. Lanza, M. West, K. Campbell, eds). Academic Press (submitted 7/18/2012).
RS Prather, M. Lorson, J.W. Ross, J. Whyte, E. Walters. 2013. Genetically engineered pig models for human diseases. In: Annual Review of Animal Biosciences, Vol. 1 (L. Andersson, D.E. Bauman, W.C. Brown, H.A. Lewin, S.J. O’Brien, E.A. Ostrander, R.M. Roberts, L.J. Saif, G.E. Seidel, Jr., A. Xu, Eds.). Annual Reviews, Palo Alto, CA. (Proofed 7/18/2012).
RS Prather. 2013. Pig genomics for biomedicine. Nat Biotechnol. 31(2):122-4. PMID: 23392511
K Lee, BK Redel, L Spate, J Teson, AN Brown, KW Park, E Walters, M Samuel, CN Murphy, RS Prather. 2013. Piglets produced from cloned blastocysts cultured in vitro with GM-CSF. Mol Reprod Dev. 80(2):145-54. PMID: 23239239
EM Walters, E Wolf, JJ Whyte, J Mao, S Renner, H Nagashima, E Kobayashi, J Zhao, KD Wells, JK Critser, LK Riley, RS Prather. 2012. Completion of the swine genome will simplify the production of swine as a large animal biomedical model. BMC Med Genomics. 5:55. PMID: 23151353
X Yang, J Mao, EM Walters, M Zhao, J Teson, K Lee, RS Prather. 2012. Xenopus Egg Extract Treatment Reduced Global DNA Methylation of Donor Cells and Enhanced Somatic Cell Nuclear Transfer Embryo Development in Pigs. Biores Open Access. 1(2):79-87. PMID 23515109
J Mao, K Tessanne, KM Whitworth, LD Spate, EM Walters, MS Samuel, CN Murphy, L Tracy, J Zhao, RS Prather. 2012. Effects of combined treatment of MG132 and scriptaid on early and term development of porcine somatic cell nuclear transfer embryos. Cell Reprogram. 14(5):385-9. PMID: 22917492
H. Men, EM Walters, H Nagashima, RS Prather. 2012. Emerging applications of sperm, embryo and somatic cell cryopreservation in maintenance, relocation and rederivation of swine genetics. Theriogenology. Nov;78(8):1720-9. PMID: 22898022
J Mao, KM Whitworth, LD Spate, EM Walters, J Zhao, RS Prather. 2012. Regulation of oocyte mitochondrial DNA copy number by follicular fluid, EGF, and neuregulin 1 during in vitro maturation affects embryo development in pigs. Theriogenology. 78(4):887-97. PMID:22626782
JW Ross, JP Fernandez de Castro, J Zhao, M Samuel, EM Walters, C Rios, P Bray-Ward, BW Jones, RE Marc, W Wang, L Zhou, J Noel, M McCall, P DeMarco, RS. Prather, H J. Kaplan. Generation of an inbred miniature pig model of retinitis pigmentosa. 2012. Investigative Ophthalmology & Visual Science. 2012. IVOS. 53(1):501-507. PMID: 22247487
EM Walters, Y Agca, V Ganjam, T Evans. Animal Models Got you Puzzled? Think Pig. 2011. Annuals of The New York Academy of Sciences. PMID: 22211982
Richards MP, Kathirvel P, Gong Y, Lopez-Hernandez A, Walters EM, Prather RS. Long chain omega-3 fatty acid levels in loin muscle from transgenic (fat-1 gene) pigs and effects on lipid oxidation during storage. 2011. Food Biotechnology 25:103-114.
Hongsheng Men, Chongbei Zhao, Si Wei, Clifton N. Murphy, Martha W. Bennett, August Rieke, Lee Spate, Yang Liu, Eric M. Walters, Melissa S. Samuel, Randall S. Prather, John K. Critser. Birth of piglets from in vitro produced, zona-intact porcine embryos vitrified in a closed system. 2011. 76(2)280-9. Theriogenology. PMID 21458047.
Whyte, J.J., R.S. Prather. 2011. Genetic modifications of pigs for medicine and agriculture. Molecular Reproduction and Development 78:879-891. NIHMSID #299529
Whyte, J.J., R.S. Prather. 2012. Zinc-finger nucleases to create custom-designed modifications in the swine (sus scrofa) genome. J. Animal Science (accepted 10/24/2011). PMID 22038991
Whyte JJ, Samuel MS, Mahan E, Padilla J, Simmons GH, Arce-Esquivel AA, Bender SB, Whitworth KM, Hao YH,
Murphy CN, Walters, EM, Prather, RS, and Laughlin, MH. 2011. Vascular endothelium-specific overexpression of
human catalase in cloned pigs. Transgenic Res. [epub ahead of print].
Zhao J, Whyte JJ, and Prather RS. 2010. Effect of epigenetic regulation during swine embryogenesis and on cloning
by nuclear transfer. Cell and tissue research 341:13-21. PMID: 20563602
Men H, Zhao C, Wei S, Murphy CN, Spate L, Liu Y, Walters EM, Samuel MS, Prather RS, Critser JK. Birth of piglets from in vitro-produced, zona-intact porcine embryos vitrified in a closed system. Theriogenology. 2011 Mar 30
Whyte JJ, Zhao J, Wells KD, Samuel MS, Whitworth KM, Walters EM, Laughlin MH, and Prather RS. 2011a. Gene
targeting with zinc finger nucleases to produce cloned eGFP knockout pigs. Mol. Reprod. Dev. 78:2. PMID 21268178
Zhao, J., Y. Hao, J.W. Ross, L.D. Spate, E.M. Walters, M.S. Samuel, A. Rieke, C.N. Murphy,R.S. Prather 2010. Histone deacetylase inhibitors improve in vitro and in vivo developmental competence of somatic nuclei nuclear transfer porcine embryos. Cellular Reprogramming 12:75-83. PMCID: PMC2842950.
Ross, J.W., J.J. Whyte, J. Zhao, M. Samuel, K.D. Wells, R.S. Prather. 2010. Optimization of square-wave electroporation for transfection of porcine fetal fibroblasts. Transgenic Research 19:611-620. PMCID: PMC2894267.
Whitworth, K.M., R.S. Prather. 2010. Somatic cell nuclear transfer efficiency- Remodeling and reprogramming: How can it be improved? Molecular Reproduction & Development 77:1001-1015. PMID 20931660
X Yang, J Mao, EM Walters, M Zhao, K Lee, RS Prather. 2012. Treatment of donor cells with Xenopus oocyte extract enhanced SCNT embryo development. 38th Annual International Embryo Transfer Society. Phoenix, AZ.
J Mao, KM Whitworth, LD Spate, EM Walters, J Zhao, RS Prather. 2012. Supplementation of maturation medium with follicular fluid, Epidermal Growth Factor, and Neuregulin affects mitochondrial DNA replication, oocyte maturation, and embryo development in pigs. 38th Annual International Embryo Transfer Society. Phoenix, AZ.
EM. Walters, MS. Samuel KD Wells, LK Riley, Randall S Prather. 2012. The Pig as a Biomedical Model-How the National Swine Resource and Research Center Can Help You. 38th Annual International Embryo Transfer Society. Phoenix, AZ. PMID:22394972
E. M. Walters, T.J Evans, Y. Agca, V.K. Ganjam. 2011. Development of a Porcine Model for Exposure to Endocrine Disrupting Chemicals. Animal Models and Their Value in Predicting Drug Efficacy and Toxicity. New York Academy of Sciences. New York City, New York.
Prather, R.S. 2011. Technical blocks to efficient pig cloning. CTS-IXA 2011 Joint International Congress, Miami, FL. Oct. 23-26.
Whyte, J.J., J. Zhao, K.D. Wells, M.S. Samuel, K.M. Whitworth, E.M. Walters, M.H. Laughlin, R.S. Prather. 2011. Zinc-finger nucleases: new innovations in custom-designed modification of the swine genome. Swine in Biomedical Research Conference July 17-19, Chicago, IL. P 27.
Beaton, B.P., E. Walters, R.S. Prather, K. Wells. 2011. Alpha-Galactosyltransferase (a1,3-Gal) Knockout Genetically Linked to Xenograft Transgenes. Swine in Biomedical Research Conference July 17-19, Chicago, IL. P 28.
Li, C., C. O’Gorman, R.S. Prather, J.A. Green, K.D. Wells. 2011. BWS symptoms in cloned piglets are associated with hypomethylation at the KCNQ-OT1 CpG island. Swine in Biomedical Research Conference July 17-19, Chicago, IL. P 35.
O’Gorman, C.W.. J. Zhao, M.S. Samuel, E.M. Walters, R.S. Prather, P. Sutovsky, M. Sutovsky, K.D. Wells. 2011.Swine in Biomedical Research Conference July 17-19, Chicago, IL. P 36.
Zhao, J.G., E.M. Walters, J. Calcaterra, L. Kelso, K. Giroux, M.S. Samuel, S. Butler, W.H. Velander, R.S. Prather. 2011. Increased propeptide processing of Factor IX in the milk of transgenic pigs by co-expression of Furin. Swine in Biomedical Research Conference July 17-19, Chicago, IL. P 41.
Whyte, J.J., R.S. Prather. 2011. Zinc-Finger Nucleases: New Innovations in Custom-Designed Modification of the Swine Genome. ADSA-ASAS Annual Meeting.
Whitworth, K. J. Teson, K. Lee, J. Mao, K. Tessanne, L.D. Spate, R.S. Prather. 2012. The histone deacetylase inhibitor 4-iodo-Suberoylanilide Hydroxamic Acid (I-SAHA) improves total cell number in pig nuclear transfer blastocysts. International Embryo Transfer Society Annual Meeting. Reprod. Fertil. & Devel. 24: 125.
Tessanne, K., B. Bauer, K. Whitworth, L. Spate, A. Brown and R.S. Prather. 2012. Characterization of the PRMT-DDAH-NO axis during porcine embryo development International Embryo Transfer Society Annual Meeting. Reprod. Fertil. & Devel. 24:185.
JJ Whyte, SC Isom, WG Spollen, SM Blake, EM Walters, RS Prather. 2011. DNA methylome analysis in nuclear transfer cells and its relationship to cloning efficiency in swine. 37th Annual International Embryo Transfer Society. Orlando, FL.
C. O’Gorman, J. Zhao, M Samuel, E. Walters, R. Prather, P. Sutovsky, M. Sutovsky, K.D. Wells.2011. Construction of a genetically engineered pig expressing GFP-labeled proteasomes. 37th Annual International Embryo Transfer Society. Orlando, FL.